Journal of orthopaedic surgery and research nanosecond pulsed electric field inhibits proliferation and induces apoptosis in human osteosarcoma xudong miao 0 3 shengyong yin 0 1 zhou shao 1 yi zhang 2 xinhua chen 1 0 equal contributors 1 the department of hepatobiliary and pancreatic surgery, the first affiliated hospital, zhejiang university, collaborative innovation center for. To further identify the antitumor function of nspef, we performed nspef experiment in the ectopic tumorbearing mice model. Pdf nanosecond pulsed electric field nspef effects on cells and. Spefr checklist of things to do before the student arrives. This article on a gene on human chromosome 20 is a stub. In this study, we sought to determine more specifically the effects of nspef on cell structure and function by exposing human cells to nspef and analyzing them at the cellular and molecular levels.
A specialized setup for nspef exposure of individual cells in. Nspef ablation of cancer targets at least three hallmarks of cancer evasion. Pdf pulse power technology using high intensity up to 300 kvcm nanosecond pulsed electric. Fitting the second region with a logarithmic function fig. Pdf nanosecond, highintensity pulsed electric fields. The 95% confidence interval for ld 50 was determined by calculation of a 95% confidence interval corridor range for the best fit shown by shaded areas in fig. The spefr at a glance student practice evaluation form. Nanosecond pulsed electric fields modulate cell function. Nanosecond pulsed electric fields nspefs can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. Nanosecond pulsed electric fields nspefs regulate phenotypes. R e s u l t s a n d d is c u s s io n pre vious publications describe important aspects of the beha vior of the ep local models, assigned to the tw o systems 733. The laboratory follow up of infection and liver function post nspef treatment because the hydatid cysts in our study is the ce1 type cysts, the complete cyst wall protects the inner parasite antigens from the hosts immune system, serological test on immunological antibodies against specific parasite antigens such as protoscolex and cyst fluid were negative. In this study, we found that after nspef application, the fluorescence lifetime became. Nspef can generate pulsed high voltage electric field in ultra short nanosecond.
To determine more specifically their effects on cell structure and function, human cells were exposed to high intensity up to 300 kvcm nanosecond 10300 ns pulsed electric fields nspef and. Chen r, chen x, beebe sj 20 nanosecond pulsed electric field. The power query m function reference includes articles for each of the over 700 functions. Cal pains, which are activated by nspefs 20,30, have been. Effects of nanosecond pulsed electric fields on the intracellular. Sperm contain cilia, and a mutation in the spef2 gene can cause male infertility due to immobile sperm.
An r function is a packaged recipe that converts one or more inputs called arguments into a single output. Nanosecond pulsed electric field nspef has emerged as a promising tool for hepatocellular carcinoma ablation recently. Verbridge3, 1department of mechanical engineering, 2department of human nutrition, foods, and exercise, and 3department of biomedical engineering. These studies describe the effects of nanosecond 10300 ns pulsed electric fields nspef on mammalian cell structure and function. Nanosecond pulsed electric fields nspefs have a variety of applications in the biomedical and biotechnology industries. In fact, many of the functions in r are actually functions of functions. We investigated the effects of nanosecond pulsed electric fields nspef on three human cell lines and demonstrated breakdown of the cytoskeleton, nuclear membrane and chromosomal telomere damage. Becoming familiar with the spef r overview of the spef r package.
Functions for fitting semiparametric regression models for panel count survival data. Since the antitumor function of nspefs has been validated, we. You can use it for descriptive statistics, generalized linear models, kmeans clustering, logistic regression, classification and regression trees, and decision forests. Navigating the spef r the language of the spef r other components of the spef r. In order to do this, state space model of the spv system with vsg controller has been developed. Sperm flagellar protein 1 is a protein that in humans is encoded by the spef1 gene. Function spef for the system under consideration have also been analyzed for the same purpose. To study the correlation between nspef induced antitumor immunity and the specific cell death pathways. Pdf mechanisms and immunogenicity of nspefinduced cell. In this paper, we describe distinct effects of nspef that appear to be largely independent of effects on the outer cell membrane, suggesting the potential to modulate cell function by circumventing the plasma membrane and effecting cell structures and functions from inside the cell. Wangyans augmented estimating equations aee, aeex, huangwangzhangs method hwz, zhangs maximum. Mechanisms and immunogenicity of nspefinduced cell death. Revised for occupational therapy university of queensland.
A causative nonsense mutation was validated by western blot analysis, strongly suggesting that the bgh phenotype results from the loss of spef2 function. Cardiac myocyte excitation by ultrashort highfield pulses. Experimental nanopulse ablation of multiple membrane. The format is ts vector, start, end, frequency where start and end are the times of the first and last observation and frequency is the number of observations per unit time 1annual, 4quartly, 12monthly, etc. However, little is known about how nspef affects liver regeneration while being applied to eliminate liver lesions. On basis of the in vitro experiment, nspef could induce cell apoptosis, inhibit cell proliferation, and inactivate metastasis and invasion. Request permission export citation add to favorites track citation. Besides, the impact of nspef ablation on liver function should also be taken into consideration in the process. In a pig animal model, a spef2 mutation affects the sperm tail development.
Nanosecond pulsed electric field nspef ablation as an. Multiple pulses enhanced permeabilization, for example, r m in gh3 cells fell. Spef2 plays an important role in spermatogenesis and flagellar assembly. Fits semiparametric regression models for panel count survival data. Nanosecond pulsed electric fields depolarize transmembrane. Nanosecond pulsed electric fields enhanced chondrogenic. Effects of nanosecond pulsed electric fields nspef on human prostate cancer cell line lncap. The recipe is implemented as a single r expression that uses the values of the arguments to compute the result. The impact of ultrashort nanopulse on cellular membrane is of biological significance and thus has been studied intensively. To learn more about functions and how they work in an expression, see understanding power query m functions. Documents from the spefr package developed by the division of occupational therapy, school of health and rehabilitation sciences, the university of queensland should be referenced as follows. Using a function as one of the arguments in a formula that uses a function is called nesting, and well refer to that function as a nested function.
Although the function of nspefs has been explored in multiple cell lines, including tlymphocytes cell lines, hematologic cell lines and pancreatic. This site is designed for professional practice educators using the spef r. Taken together, the data in this study demonstrate that spef2 is required for cilia function and identify a new genetic cause of pcd. An overview of the package can be found in wang and yan 2011 spef. Cancer treatment has been at the forefront of investigations thus far as nspefs permeabilize cellular and intracellular membranes leading to apoptosis and necrosis. Influence of pulsed electric fields and mitochondria. Dielectrophoresisdep of spherical cells after nano.
One of the great strengths of r is the users ability to add functions. The ts function will convert a numeric vector into an r time series object. However, little is known about their effects on the differentiation of stem cells. Different from cell study, this ex vivo study aims to investigate the biological effects of nanosecond pulsed electric field nspef on an independent multimembrane parasite, human hydatid cyst, to observe the unique influence of nanopulse on macromembrane structure. Selective susceptibility to nanosecond pulsed electric. Excel for microsoft 365 excel for the web excel 2019 excel 2016 excel 20 excel 2010 excel 2007 more. Nsepy is a library to extract historical and realtime data from nses website. Spef has then been derived with this model for a spv source connected to a synchronous generator sg by a cable with the nonlinear load. Tmem16f has been reported to function as a swellingactivated cl 38, outwardly rectifying cl 39, and ca2activated cl channel 4043. Novel interventional management of hepatic hydatid cyst. Modulation of apoptosis regulation has recently shown therapeutic promise in the treatment of some diseases, including cancer 31. The ire pulse example is an idealized trapezoidal w aveform. Navigating the spefr the language of the spefr other components of the spefr package. Impact of vsg parameters on transient performance of solar.
F or the nspef example we used an experimental pulse w aveform 4. Making the most of the spef r trends in sound assessment practice. As the pulse durations decrease, effects on the plasma membrane pm decrease and effects on intracellular signal transduction mechanisms increase. Nanosecond pulsed electric field nspef treatment for. Application the fault indicator type spef 3a2 c is used in association with disconnector terminal units dtus for detection of short circuits, earth faults and phase discontinuity in distri bution networks. The revoscaler library is a collection of portable, scalable, and distributable r functions for importing, transforming, and analyzing data at scale. Mice bearing 4050 mm 3 b16f10 t umors were treated with either 500 or 750, 200ns pulses 25 kvcm. Cells can be driven into cell death subroutines that include necrotic features 28,29. Nanosecond pulsed electric field inhibits cancer growth. Spef2 is expressed in all ciliated cells and is required for cilia function. The fault indicator functions a data acquisition unit which allows measured information and. Python is a great tool for data analysis along with the scipy stack and the main objective of nsepy is to provide analysis ready dataseries for use with scipy stack. The laboratory follow up of infection and liver function post nspef treatment. Beebe1,3 1 frank reidy research center for bioelectrics, old dominion university, norfolk, va, usa 2 department of.
Nanosecond pulsed electric field induced cytoskeleton. Undoubtedly, cells respond to nspefs in diverse and celltypespecific ways. These results have been obtained under different experimental conditions, which may explain. Nspef ablated primary b16f10 melanomas and protected 33% of the animals from tumor challenge. Pulse power technology using nanosecond pulsed electric fields nspefs offers a new stimulus to modulate cell functions or induce cell death for cancer cell ablation. Upregulation of pdgf mediates robust liver regeneration. Dualfunction of baicalin in nspefstreated hepatocytes and. Mechanisms of nanosecond pulsed electric field nspefinduced. The effects of pulsed electric fields have been a period of study since past few decades, which was mainly on the electroporation, bacterial decontamination and biofouling. Nanosecond pulsed electric field nspef longdom publishing sl.